Which of the following is not a cloning vector?

QUESTION: Which of the following is not a cloning vector?

A Cosmid

B  PBR 322

C  Sall

D  Phagemid

ANSWER:    Sall

 

Vector Nature                Description Role in Cloning Process
Cosmid Cloning vector ·        Cosmids are hybrid vectors that combine the features of plasmids and bacteriophages.

·        They can carry larger DNA inserts compared to traditional plasmids. Cosmids contain an origin of replication for E. coli and a phage DNA packaging signal (cos site). These features allow cosmids to be packaged into phage particles and propagated in E. coli.

 ·        Cosmids are utilized for cloning larger DNA fragments that might not fit into regular plasmids, enabling the study of larger genetic elements or entire genes.

·        They are especially useful for genomic libraries and mapping studies.
 pBR322 Cloning vector ·        PBR322 is a small, circular DNA plasmid that was one of the earliest widely used cloning vectors.

·        It carries genes for antibiotic resistance (ampicillin and tetracycline) and a multiple cloning site (MCS).

·        The MCS contains multiple unique restriction sites for inserting DNA fragments.

 ·        PBR322 is employed for cloning small to moderate-sized DNA fragments.

·        It is a versatile vector suitable for general cloning, DNA sequencing and protein expression.

·        Its antibiotic resistance markers aid in selection and maintenance of transformed bacteria.
Phagemid Cloning vector ·        Phagemids are hybrid vectors combining features of both plasmids and filamentous bacteriophages.

·        They have an origin of replication for E. coli and a phage origin of replication.

·        Phagemids can replicate as plasmids within bacterial cells or produce phage particles that encapsulate single-stranded DNA.

 ·        Phagemids are used for cloning and expression of DNA sequences.

·        They can be easily propagated as plasmids or as phage particles, allowing for the production of single-stranded DNA for sequencing or for generating DNA libraries.
 Sall

 

 

 

 

 

 Enzyme

 

 

 

 

 

 ·        Sall is a type II restriction enzyme that recognizes the DNA sequence 5′-GTCGAC-3′.

·        It cleaves the DNA at this specific site.

 

 

 

 

 ·        Sall is not a cloning vector but rather an enzyme used in the digestion of DNA during cloning procedures.

·        It helps to generate compatible ends for ligation when inserting DNA fragments into vectors that have been linearized with the same enzyme.

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