[OBJECTIVE]
Subject: Gene Cloning
Time Allowed: 15 Minutes
Maximum Marks: 10
NOTE: Attempt this Paper on this Question Sheet only. Please encircle the correct option. Division of marks is given in front of each question. This Paper will be collected back after expiry of time limit mentioned above.
Part-I Encircle the right answer, cutting and overwriting are not allowed. (10)
1. mRNA can be converted into DNA by
a. Hybrid DNA
b. Taq DNA Polymerase
c. Ribosomal DNA
d. Complementary DNA
2. In PCR procedure when DNA hydrogen bonds are broken at 94 °C is called ________.
a. Annealing
b. Denaturation
c. Synthesis
d. None
3. At the end of a PCR a sample of the reaction mixture is usually analysed by
a. DNA sequencing
b. Gene Cloning
¢. Gel electrophroses
d. Southern Hybridization
4. _________DNA consists of all the DNA present in a single cell or group of cells.
a. Hybrid
b. Ribosomal
c. Total
d. Complementary
5. A DNA sequence that is able to move from place to place within a genome.
a. Template
b. Transposon
c. Transcript
d. Terminator
6. If two plasmids are unable to live in the same bacterial cell they are said to be ________________.
a. Compatible
b. Conjugative
c. Incompatible
d. None
7. Ethidium bromide is used as a dye to stain DNA in agarose and polyacrylamide gel in following.
a. Blotting
b. Finger prints
c. Electrophoresis
d. None
8. Ti plasmid can cause _______ disease.
a. Hairy root
b. Proliferation
c. Crown gall
d. Both a and c
9. A virus with an RNA genome is _____________.
a. Plasmid
b. Episome
c. Retro virus
d. None
10. A thermostable DNA polymerase that is used in PCR is _________.
a. Taq DNA polymerase
b. DNA Ligase
c. DNA Polymerase III
d. DNA Topoisomerase
[SUBJECTIVE]
Subject: Gene Cloning
Time Allowed: 2 Hours 45 Minutes
Maximum Marks: 50
NOTE: ATTEMPT THIS (SUBJECTIVE) ON THE SEPARATE ANSWER SHEET PROVIDED.
Part-II Give short notes on following, each question carries equal marks. (20)
Q#1: What is the function of yeast artificial chromosome (YAC)?
Q#2: What is the difference between transfection and transformation?
Q#3: How 5’-terminus end is different from 3′-terminus end?
Q#4: What are exons?
Q#5: Explain the purpose of Western transfer.
Q#6: Give the significance of lambda vector in gene cloning.
Q#7: What are linkers?
Q#8: What is restriction map?
Q#9: What is the purpose of polymerase chain reaction (PCR)?
Q#10: What are the advantages of high copy number of plasmid in gene cloning?
Part-III Give detailed answers, each question carries equal marks. (30)
Q#1: What do you know about role of vectors in gene cloning?
Q#2: What are the basic features of Bacteriophages? Explain the lysogenic cyle of phage virus.
Q#3: Explain briefly about Lambda phage as cloning vector.
Q#4: How Ti plasmid cause crown gall disease in plants? Explain briefly.
Q#5: Write a note on PCR.
Q#6: Explain the role of gene cloning in Agriculture.