Enzymes MCQs

By: Prof. Dr. Fazal Rehman | Last updated: May 22, 2025

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1. : Aldolase belongs to ________ class of enzymes.



2. : All of the following enzymes show group specificity, excluding



3. : Pyruvate carboxylase belongs to ________ class of enzymes.



4. : The co-enzymes have which properties?



5. : When substrate concentration is equal to Km of an enzyme:



6. : A sigmoidal plot of substrate concentration ([S]) versus reaction velocity (V) may indicate:



7. : An inducer is not needed in which type of enzyme?



8. : The V of an enzyme represents which of the following?



9. : Which is characteristic of a competitive inhibitor?



10. : Enzymes which are produced inactive form in the living cells are called:



11. : Which is a proenzyme?



12. : The enzyme _______ that adds water across a double bond or removes water to create a double bond without breaking the bonds?



13. : Effect of raising the temperature from 20-80°C on enzyme activity would be:



14. : Cytochrome P-450 carries which type of enzyme?



15. : What is the most ideal statement regarding the ‘Active Site’ of an enzyme?



16. : Which of the following is an example of multifunctional enzyme?



17. : An enzyme that converts an aldose sugar to a keto-sugar is classified as ______.



18. : If the initial velocity of an enzymatic reaction is equal to Vmax, the substrate concentration is:



19. : During competitive inhibition, the inhibitor:



20. : Competitive inhibitors ______.



21. : Which is a therapeutic enzyme?



22. : The role of co-enzyme in enzymatic reactions is:



23. : Which is not true regarding enzymes?



24. : Michaelis constant (Km) of an enzyme is ______.



25. : During the estimation of enzyme activity, all of the following are kept constant, excluding:



26. : As per Michaelis-Menten equation, at a very low substrate concentration, the initial velocity of the enzymatic reaction is ________.



27. : The isoenzymes differ from each other in all of the following, excluding:



28. : What is Lock and Key Theory?



29. : Which was the first enzyme to be isolated, purified, and crystallized?



30. : The concept of “Induced Fit” as a theory implies which of the following?



31. : The alloenzymes are



32. : Amil Fischer’s ‘Lock-and-key’ theory for enzymes was supported by the fact—



33. : All _________ enzymes carry Flavin nucleotides as prosthetic group.



34. : Which type of enzyme can supply the citric acid cycle with oxaloacetate from pyruvate?



35. : Cooperativity of an allosteric enzyme can be studied by—



36. : In-vivo control of an enzymatic reaction can be achieved by:



37. : Which is a co-enzyme?



38. : Hydrolysis of proteins by protease enzymes would yield amino acids in the form of which of the following?



39. : A number of biochemical assays are linked with enzymatic conversion of NAD/NADH. The reaction velocity in these assays is determined by measuring the change in optical density at:



40. : The Vmax of an enzymatic reaction may be dependent on which of the following?



41. : Which is a hydrogen transferring co-enzyme?



42. : According to steady state kinetics, which statement is false?



43. : Mostly the regulatory reactions in a pathway are enzyme limited. Which is true about such reactions?



44. : The enzymes ______ are always present in a cell/organism at relatively constant concentrations.



45. : Which among the following is extracellular?



46. : Choose the most ideal statement regarding allosteric enzymes:



47. : The isoenzymes are:



48. : Which enzymes catalyze the binding of two substrates by covalent bonds?



49. : Who proposed the induced fit model of enzyme action?



50. : In Lineweaver-Burk plot, the Y-intercept represents:



51. : Which statement regarding enzyme kinetics is NOT TRUE?



52. : Which is NOT TRUE regarding enzymes?



53. : Which is NOT a characteristic of allosteric enzymes?



 

Important Questions about Enzymes

  1. Enzymes MCQs
  2. Inhibition in enzymes
  3. Which enzyme digests starch?
  4. Which enzyme digests protein?
  5. which enzyme is present in saliva?
  6. Which enzyme is not present in succus entericus?
  7. Which enzyme breaks down protein in the stomach?
  8. Which enzyme converts fibrinogen to fibrin?
  9. Which enzyme converts glucose into alcohol?
  10. Which enzyme digests fat?
  11. Which enzyme digests milk protein?
  12. Which enzyme is used in PCR?
  13. Which enzyme unzips the DNA double helix?
  14. Which liver enzyme increases with alcohol?
  15. Which enzyme cuts DNA?
  16. Which enzyme catalyzes the first step of glycolysis?
  17. Which enzyme binds DNA fragments together?
  18. Which enzyme transcribes DNA into RNA?
  19. which enzyme adds new nucleotides?
  20. Which enzyme digests cellulose?
  21. Which enzyme separates the two strands of DNA?
  22. Which enzyme converts carbohydrates into glucose?
  23. Which enzyme removes RNA primer?
  24. Which enzymes help in digestion?
  25. Which enzyme works best in acidic conditions?
  26. Which enzyme synthesizes tRNA?
  27. Which enzyme secreted by pancreas?
  28. Which enzyme converts maltose into glucose?
  29. Which vitamins work as coenzyme?

More Biochemistry MCQs

  1. Acid and Base MCQs
  2. Carbohydrate MCQs
  3. Proteins MCQs
  4. Lipids and Biomembranes MCQs
  5. Nucleic acids MCQs
  6. Metabolism of Carbohydrates MCQs
  7. Enzymes MCQs
  8. Vitamins and Hormones MCQs
  9. Bioenergetic MCQs
  10. Biotechnology MCQs
  11. Biotechnology MCQs 2
  12. Basic Biochemistry MCQs (important)
  13. CHEMISTRY MCQs From all subjects of  Chemistry

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