Q: Which enzyme is used in PCR?
ANS:
- Heat-stable DNA polymerase also known as Taq polymerase is commonly used in PCR (Polymerase Chain Reaction).
- Taq polymerase is derived from the bacterium Thermus aquaticus, which lives in hot springs and thermal vents.
- It is highly heat-resistant and can withstand the high temperatures required during the PCR process.
| Enzyme | Taq Polymerase |
| Source | Thermus aquaticus bacterium |
| Function | DNA polymerase used in PCR (Polymerase Chain Reaction) |
| Optimal Temperature | Around 72-75°C |
| DNA Synthesis | Incorporates nucleotides to extend DNA strands |
| DNA Amplification | Allows repeated cycles of denaturation, annealing, and extension |
| Application | Used in various molecular biology techniques and diagnostics |
| Commercial Availability | Widely available from multiple suppliers |
| Alternative Enzymes | Other heat-stable DNA polymerases with improved characteristics are available |
Important Questions about Enzymes
- Enzymes MCQs
- Inhibition in enzymes
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- Which enzyme is used in PCR?
- Which enzyme unzips the DNA double helix?
- Which liver enzyme increases with alcohol?
- Which enzyme cuts DNA?
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- Which enzyme transcribes DNA into RNA?
- which enzyme adds new nucleotides?
- Which enzyme digests cellulose?
- Which enzyme separates the two strands of DNA?
- Which enzyme converts carbohydrates into glucose?
- Which enzyme removes RNA primer?
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- Which enzyme works best in acidic conditions?
- Which enzyme synthesizes tRNA?
- Which enzyme secreted by pancreas?
- Which enzyme converts maltose into glucose?
- Which vitamins work as coenzyme?