Question: Which enzyme binds DNA fragments together?
Answer:
- DNA ligase is an enzyme that binds DNA fragments
- Ligase plays a crucial role in
- DNA replication,
- Repair
- Recombination processes
- It catalyzes the formation of phosphodiester bonds between adjacent DNA fragments.
- When DNA is damaged or during DNA replication, DNA strands can become broken or contain gaps. DNA ligase repairs these breaks by sealing the gaps and joining the DNA fragments together.
| Enzyme | DNA Ligase |
| Function | Catalyzes the formation of phosphodiester bonds between adjacent DNA fragments |
| Substrate | Requires a nick or a gap in the DNA backbone to join the fragments |
| DNA Repair | DNA ligase is involved in various DNA repair processes, including base excision repair and nucleotide excision repair |
| Types | Different types of DNA ligases exist, including DNA ligase I, DNA ligase III, and DNA ligase IV |
| Role in Replication | DNA ligase plays a vital role in the lagging strand synthesis during DNA replication, joining Okazaki fragments |
| Recombinant DNA | DNA ligase is used in recombinant DNA technology to join DNA fragments from different sources |
| PCR | In PCR (Polymerase Chain Reaction), DNA ligase can be used to join DNA adapters or linkers to target DNA |
| Storage | DNA ligase is typically stored at low temperatures, often at -20°C or -80°C |
| Allosteric Regulation | DNA ligase activity can be modulated by factors such as ATP concentration and other cofactors |
| Clinical Significance | Mutations in DNA ligase genes can lead to rare genetic disorders, such as DNA ligase deficiency |
| Molecular Biology Applications | DNA ligase is widely used in molecular biology techniques, including cloning, gene expression, and genetic engineering |
| Commercial Availability | DNA ligase enzymes are commercially available from various suppliers |
Important Questions about Enzymes
- Enzymes MCQs
- Inhibition in enzymes
- Which enzyme digests starch?
- Which enzyme digests protein?
- which enzyme is present in saliva?
- Which enzyme is not present in succus entericus?
- Which enzyme breaks down protein in the stomach?
- Which enzyme converts fibrinogen to fibrin?
- Which enzyme converts glucose into alcohol?
- Which enzyme digests fat?
- Which enzyme digests milk protein?
- Which enzyme is used in PCR?
- Which enzyme unzips the DNA double helix?
- Which liver enzyme increases with alcohol?
- Which enzyme cuts DNA?
- Which enzyme catalyzes the first step of glycolysis?
- Which enzyme binds DNA fragments together?
- Which enzyme transcribes DNA into RNA?
- which enzyme adds new nucleotides?
- Which enzyme digests cellulose?
- Which enzyme separates the two strands of DNA?
- Which enzyme converts carbohydrates into glucose?
- Which enzyme removes RNA primer?
- Which enzymes help in digestion?
- Which enzyme works best in acidic conditions?
- Which enzyme synthesizes tRNA?
- Which enzyme secreted by pancreas?
- Which enzyme converts maltose into glucose?
- Which vitamins work as coenzyme?