Question: Which enzyme cuts DNA?
Answer:
- Restriction endonucleases, also known as restriction enzymes, are responsible for cutting DNA molecules at specific recognition sites.
- These enzymes are naturally occurring in bacteria and are used as a defense mechanism against invading viral DNA.
- Restriction enzymes recognize specific DNA sequences and cleave the DNA at those sites.
- Examples of commonly used restriction enzymes include
- EcoRI
- HindIII
- BamHI
- These enzymes are named after the bacteria from which they were initially isolated.
| Enzyme | Restriction Enzymes |
| Function | Recognize specific DNA sequences and cleave the DNA at or near those sites |
| Types | Hundreds of different restriction enzymes with unique recognition sequences |
| Cleavage Patterns | Can produce either blunt ends or sticky ends upon DNA cleavage |
| Recognition Sites | Enzymes have specific recognition sequences of 4 to 8 base pairs |
| Sticky Ends | DNA is cut in a staggered manner, leaving short overhanging single-stranded regions |
| Palindromic | Recognition sequences are often palindromic (read the same forward and backward) |
| DNA Manipulation | Allow for the precise manipulation of DNA molecules by inserting, deleting, or rearranging specific DNA sequences |
| Commercial Availability | Restriction enzymes are commercially available from various suppliers |
| Isoschizomers | Different enzymes that recognize the same recognition sequence |
| Methylation Sensitivity | Some restriction enzymes are sensitive to DNA methylation, while others are not |
| Storage | Restriction enzymes are typically stored at low temperatures, often at -20°C or -80°C |
| Applications | Widely used in molecular biology techniques, such as DNA cloning, genetic engineering, and DNA analysis |
Important Questions about Enzymes
- Enzymes MCQs
- Inhibition in enzymes
- Which enzyme digests starch?
- Which enzyme digests protein?
- which enzyme is present in saliva?
- Which enzyme is not present in succus entericus?
- Which enzyme breaks down protein in the stomach?
- Which enzyme converts fibrinogen to fibrin?
- Which enzyme converts glucose into alcohol?
- Which enzyme digests fat?
- Which enzyme digests milk protein?
- Which enzyme is used in PCR?
- Which enzyme unzips the DNA double helix?
- Which liver enzyme increases with alcohol?
- Which enzyme cuts DNA?
- Which enzyme catalyzes the first step of glycolysis?
- Which enzyme binds DNA fragments together?
- Which enzyme transcribes DNA into RNA?
- which enzyme adds new nucleotides?
- Which enzyme digests cellulose?
- Which enzyme separates the two strands of DNA?
- Which enzyme converts carbohydrates into glucose?
- Which enzyme removes RNA primer?
- Which enzymes help in digestion?
- Which enzyme works best in acidic conditions?
- Which enzyme synthesizes tRNA?
- Which enzyme secreted by pancreas?
- Which enzyme converts maltose into glucose?
- Which vitamins work as coenzyme?