Engineering Chemistry MCQs on Chromatography 

By: Prof. Dr. Fazal Rehman | Last updated: November 23, 2024

1. How the Purification of enzymes and proteins is done?
(A). By using the Affinity chromatography
(B). By using the Liquid chromatography
(C). By using the Column chromatography
(D). By using the Thin layer chromatography
(E). None of these
Multiple Choice Question Answer: a

2. The Affinity chromatography is used for the analysis and isolation of which of the following?
(A). Insoluble starch substances
(B). Enzyme tyrosinase
(C). None of these
(D). Antibodies bound with a covalently-attached antigen on cellulose column
(E). All of these
Multiple Choice Question Answer: e

3. How the analysis and separation of industrial products like soap and synthetic detergents is done?
(A). By using the Gas chromatography
(B). By using the Partition chromatography
(C). By using the Ion exchange chromatography
(D). By using the Thin layer chromatography
(E). None of these
Multiple Choice Question Answer: b

4. By the application of thin layer chromatography we can check purity of samples.
(A). True
(B). False
(E). None of these
Multiple Choice Question Answer: a

5. How the quantitative analysis is done?
(A). By using the Liquid chromatography
(B). By using the Thin layer chromatography
(C). By using the Ion exchange chromatography
(D). By using the Gas chromatography
(E). None of these
Multiple Choice Question Answer: d

6. The Liquid chromatography is a technique for separating the ions that are ____________.
(A). not dissolved in a solvent
(B). dissolved in a solvent
(C). dissolved in a solute
(D). All of these
(E). None of these
Multiple Choice Question Answer: b

7. The application of ion exchange chromatography is __________.
(A). None of these
(B). The demineralisation of water
(C). The separation and determination of anions
(D). The softening of hard water
(E). All of these
Multiple Choice Question Answer: e

8. What does the Chromatography involve two mutually?
(A). None of the mentioned
(B). Miscible phases
(C). Immiscible phases
(D). Soluble phases
(E). None of these
Multiple Choice Question Answer: c

9. The Chromatography is the process for identification, purification, and separation of components of a mixture on the basis of difference in their ____________.
(A). melting point
(B). affinity for mobile and stationary phase
(C). boiling point
(D). solubility
(E). None of these
Multiple Choice Question Answer: b

10. There is no need of support if packed into a column when which of the following is the stationary phase?
(A). Gas
(B). Liquid
(C). Solid
(D). None of the mentioned
(E). None of these
Multiple Choice Question Answer: c

11. Not a stationary phase is ___________.
(A). Liquid-liquid chromatography
(B). Solid-solid chromatography
(C). Gas-liquid chromatography
(D). Gas-solid chromatography
(E). None of these
Multiple Choice Question Answer: b

12. In the exclusion chromatography, solute molecules are separated based on which of the following?
(A). Molecular phase
(B). Molecular composition
(C). Molecular formula
(D). Molecular geometry and size
(E). None of these
Multiple Choice Question Answer: d

13. The gas-solid chromatography is which of the following chromatography as per basic principle involved?
(A). Adsorption
(B). Exclusion
(C). Ion-exchange
(D). Absorption
(E). None of these
Multiple Choice Question Answer: a

14. Which of the following is a proper solvent that is passed through the column for elution so that separated components can be collected?
(A). Adsorbent
(B). None of the mentioned
(C). Buffer solution
(D). Mobile phase
(E). None of these
Multiple Choice Question Answer: c

15. If the mobile phase is gas, then how the movement of solute is determined?
(A). By the Volatility
(B). By the Melting point
(C). By the Boiling point
(D). By the Solubility
(E). None of these
Multiple Choice Question Answer: a

16. The type of chromatography that is used for the structural analysis is ___________.
(A). Column chromatography
(B). Paper chromatography
(C). Partition chromatography
(D). Affinity chromatography
(E). None of these
Multiple Choice Question Answer: b

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