Research Topics Molecular biotechnology
Research Area/ Research Interest: Molecular Biotechnology
Research Paper Topics for Masters and Ph.D. Thesis and publication
- Selectable Markers to Marker-Free Selection in Rice
- Construction and Quantitative Evaluation of a Tissue-Specific Sleeping Beauty by EDL2-Specific Transposase Expression in Esophageal Squamous Carcinoma Cell …
- Identification of Optimal Expression Parameters and Purification of a Codon-Optimized Human GLIS1 Transcription Factor from Escherichia coli
- Fabrication and Molecular Modeling of Navette-Shaped Fullerene Nanorods Using Tobacco Mosaic Virus as a Nanotemplate
- Response Surface Methodology Based Optimization, Partial Purification and Characterization of Alkaline Phosphatase Isolated from Pseudomonas asiatica Strain …
- MiR-221-3p Facilitates Thyroid Cancer Cell Proliferation and Inhibit Apoptosis by Targeting FOXP2 Through Hedgehog Pathway
- Kidney-Derived Methylated PAX2 Sequences in the Urine of Healthy Subjects as a Convenient Model for Optimizing Methylation-Based Liquid biopsy
- Long Non-coding RNA ZFPM2-AS1: A Novel Biomarker in the Pathogenesis of Human Cancers
- Identification of the Talaromyces cellulolyticus Gene Encoding an Extracellular Enzyme with β-galactosidase Activity and Testing it as a Reporter for Gene Expression …
- LncRNA NEAT1 Promotes TLR4 Expression to Regulate Lipopolysaccharide-Induced Trophoblastic Cell Pyroptosis as a Molecular Sponge of miR-302b-3p
- Overexpression of Oil Palm Early Nodulin 93 Protein Gene (EgENOD93) Enhances In Vitro Shoot Regeneration in Arabidopsis thaliana
- Clinical Potential of lncRNA PPP1R26-AS1 in Breast Cancer and Its Contribution to Cancer Progression
- Bioengineering of Novel Non-Replicating mRNA (NRM) and Self-Amplifying mRNA (SAM) Vaccine Candidates Against SARS-CoV-2 Using …
- Characterization and Evaluation of Cell-Penetrating Activity of Brevinin-2R: An Amphibian Skin Antimicrobial Peptide
- Hydrogen/Deuterium Exchange Behavior During Denaturing/Refolding Processes Determined in Tetragonal Hen Egg-White Lysozyme Crystals
- Proteomic Analysis and Promoter Modification of Bacillus thuringiensis to Improve Insecticidal Vip3A Protein Production
- LncRNA MATN1-AS1 for Prediction of Prognosis in Osteosarcoma Patients and Its Cellular Function
- Structure and Function of Potential Glycosylation Sites of Dynactin-Associated Protein dynAP
- Modulation of the Immune System Promotes Tissue Regeneration
- Characterization of a Novel Shewanella algae Arginine Decarboxylase Expressed in Escherichia coli
- BMP-4 Extraction from Extracellular Matrix and Analysis of Heparin-Binding Properties
- Increasing Lovastatin Production by Re-routing the Precursors Flow of Aspergillus terreus via Metabolic Engineering
- Molecular Insights into Freezing Stress in Peach Based on Multi-Omics and Biotechnology: An Overview
- Protective Effects of Catalpol on Limb Motor Function and Ultrastructure of Hippocampal Neurons in Rats with Cerebral Ischemia
- METTL14-Mediated miR-30c-1-3p Maturation Represses the Progression of Lung Cancer via Regulation of MARCKSL1 Expression
- Reproduction ecology of an emerging fishery resource, the amphibious mudskipper Periophthalmus chrysospilos, in the Mekong Delta
- ACE2 is the critical in vivo receptor for SARS-CoV-2 in a novel COVID-19 mouse model with TNF-and IFNγ-driven immunopathology
- Biotechnology and stem cell technology overview
- CONCEPT CATEGORIES EXPRESSED IN ENGLISH TERMS OF THE BIOTECHNOLOGY SPHERE
- Project management maturity in the biotechnology industry
- Retraction Note to: Transient Activation of Reprogramming Transcription Factors Using Protein Transduction Facilitates Conversion of Human Fibroblasts Toward …
- MDContactCom: a tool to identify differences of protein molecular dynamics from two MD simulation trajectories in terms of interresidue contacts
- Cancer Immuno-prevention
- Features of the Supramolecular Cell-Genetic Organization of E. Coli in Engineering Aspects of Biotechnology
- Role of Modern Biotechnology in the Era of River Water Pollution
- Molecular approaches for improving abiotic stress tolerance in sugarcane
- Increased network centrality of the anterior insula in early abstinence from alcohol
- Genome-wide association study and transcriptome analysis reveal new QTL and candidate genes for nitrogen‐deficiency tolerance in rice
- Sensitive New Assay System for Serum Wisteria floribunda Agglutinin-Reactive Ceruloplasmin That Distinguishes Ovarian Clear Cell Carcinoma from Endometrioma
- Duck Genomics and Biotechnology
- Diversity and distribution of thermophilic microorganisms and their applications in biotechnology
- IN VITRO SCREENING AND CONFIRMATION OF MOLECULAR MARKERS IN CHICKPEA (Cicer arietinum L.) GENOTYPES FOR FUSARIUM WILT RESISTANCE …
- Amplification of human interneuron progenitors promotes brain tumors and neurological defects
- Biotechnology and its impact on future developments in soybean production and use
- Duck Genomics and Biotechnology 15
- Microalgal lipids: biochemistry and biotechnology
- New Breeding Techniques (NBTs) and Biotechnology for Boosting Rice Grain Yield to Feed 5 Billion in 2050
- Spatial components of molecular tissue biology
- Recent advances in molecular farming using monocot plants
- Machine learning and deep learning in biotechnology: applications, impacts, and challenges
- Molecular Characterization of Propionibacterium acnes Isolated from Acne vulgaris
- History and current status of sugarcane breeding, germplasm development and molecular biology in India
- Recent Advances in Molecular Techniques for the Enhancement of Crop Production
- Effects of mokF gene deletion and overexpression on the Monacolin K metabolism yields of Monascus purpureus
- The soil‐borne ultimatum, microbial biotechnology and sustainable agriculture
- Genome-wide association studies and haplotype sharing analysis targeting the growth traits in Yandang partridge chickens
- … potentiates doxorubicin sensitivity in triple‐negative breast cancer cells by targeting MDR1 and JNK and suppressing mTOR and Bcl‐2: In vitro and molecular docking …
- When microbial biotechnology meets material engineering
- Research Advances on Genetic Structure and Molecular Mechanism Underlying the Formation of Tassel Traits in Maize
- Peptide Assembled in a Nano-confined Space as a Molecular Rectifier for the Availability of Ionic Current Modulation
- Preparation and epitope mapping of monoclonal antibodies against African swine fever virus P30 protein
- Progress on Genetic Analysis and Molecular Dissection on Maize Leaf Angle Traits
- Molecular biology: Fantastic toolkits to improve knowledge and application of acetic acid bacteria
- Inhibitory Effects of 6, 8-Diprenylorobol on Endometriosis Progression in Humans by Disrupting Calcium Homeostasis and Mitochondrial Function
- A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testing
- Investigation of chetomin as a lead compound and its biosynthetic pathway
- Application of Microbes in Biotechnology, Industry, and Medical Field
- Designed SARS‐CoV‐2 receptor binding domain variants form stable monomers
- Progress and challenges in sorghum biotechnology, a multipurpose feedstock for the bioeconomy
- Latest biotechnology tools and targets for improving abiotic stress tolerance in protein legumes
- A comprehensive biotechnological and molecular insight into plastic degradation by microbial community
- Molecular Docking, Dynamics, and WaterSwap Analysis to Identify Anti-aggregating Agents of Insulin and IFN-β
- Overexpression of SRO_3163, a homolog of Streptomyces antibiotic regulatory protein, induces the production of novel cyclohexene-containing enamide in …
- … LUNATUS (Lima bean) AND ITS BIOLOGICAL APPLICATIONS P. Vishnupriya, Vardhana Janakiraman*, G. Selvamangai Department of Biotechnology, Alpha
- Hemopexin and Cancer
- Department of Biotechnology
- Big data and computational advancements for next generation of Microbial Biotechnology
- Department of Biotechnology Faculty Publication
- Glycoengineering of Darbepoetin-α in CHO-DG44 Cells through Overexpression of α-2, 3-sialyl-transferase and CMP-sialic Acid Transporter
- Genus Pseudonocardia: What we know about its biological properties, abilities and current application in biotechnology
- Blue Biotechnology: The secrets of the ocean are yet to be explored
- Current state and future prospects of biotechnology in the Russian Federation
- Extra-Intestinal Manifestations of Celiac Disease: What Should We Know in 2022?
- Characterization of Soybean Genotypes on the Basis of Yield Attributing Traits and SSR Molecular Markers
- Worldwide Protein Data Bank (wwPDB): A virtual treasure for research in biotechnology
- Effect of a Various Additivies to the Formation of Aqueous Biphasic System Polyetylenglycole (Peg)/Sodium Citrate/Water
- Ecological and molecular perspectives on responders and non-responders to probiotics and prebiotics
- Breeding and Biotechnology of Jute
- Outline of Fungi and fungus-like taxa-2021.
- Isolation and Molecular Identification of Vibrio spp. by Sequencing of 16S rDNA from Seafood, Meat and Meat Products in Libya: A Descriptive Study
- Protein-protein interactions in the regulation of the extracellular signal-regulated kinase
- Recent advances in transgenic technology
- Carotenoids and flavonoids contribute to nutritional protection against skin damage from sunlight
- An overview of structural DNA nanotechnology
- PNA technology
- Control of the G2/M transition
- Recombinant protein expression in Leishmania tarentolae
- Mutational dynamics of microsatellites
- Bioenergetic and antioxidant properties of coenzyme Q10: recent developments
- Alterations in antioxidant enzymes during aging in humans
- Identification of pathogenicity-related genes in the vascular wilt fungus Verticillium dahliae by Agrobacterium tumefaciens-mediated T-DNA insertional …
- The IUBMB-endorsed transporter classification system
- Structure and basal transcription complex of RNA polymerase II core promoters in the mammalian genome: an overview
- Anti-TNF therapy for rheumatoid arthritis and other inflammatory diseases
- Data deposition and annotation at the worldwide protein data bank
- A comparison of protein quantitation assays for biopharmaceutical applications
- Computational prediction of protein–protein interactions
- Introduction to the background, principles, and state of the art in suicide gene therapy
- Generation and production of engineered antibodies
- Differential secretion and accumulation of terpene indole alkaloids in hairy roots of Catharanthus roseus treated with methyl jasmonate
- Overexpression of StDREB1 transcription factor increases tolerance to salt in transgenic potato plants
- Multiple drug resistance mechanisms in cancer
- A new look at xylanases
- Selection of primers for polymerase chain reaction
- cDNA-AFLP analysis reveals differential gene expression in response to salt stress in foxtail millet (Setaria italica L.)
- Signaling functions of free radicals superoxide & nitric oxide under physiological & pathological conditions
- The current state of chromatin immunoprecipitation
- Resistance to tetracycline, macrolide-lincosamide-streptogramin, trimethoprim, and sulfonamide drug classes
- The biology of endotoxin
- High-yield expression of M2e peptide of avian influenza virus H5N1 in transgenic duckweed plants
- DNA methylation: an introduction to the biology and the disease-associated changes of a promising biomarker
- Data clustering in life sciences
- The ERK cascade
- Protein folding in vivo and renaturation of recombinant proteins from inclusion bodies
- With or without sugar?(A) glycosylation of therapeutic antibodies
- Identification of suitable reference genes for qRT-PCR analysis of circulating microRNAs in hepatitis B virus-infected patients
- Therapeutic uses of antioxidant liposomes
- Structural aspects of the metzincin clan of metalloendopeptidases
- Nucleic acids electrotransfer-based gene therapy (electrogenetherapy): past, current, and future
- Pluronic enhances the robustness and reduces the cell attachment of mammalian cells
- Cancer detection and treatment: the role of nanomedicines
- Adenoviral vectors for gene therapy
- Large-scale transfection of mammalian cells for the fast production of recombinant protein
- Anti-oxidants from green tea and pomegranate for chemoprevention of prostate cancer
- Polyamines
- Fundamentals of flow cytometry
- A modified protocol for RNA extraction from different peach tissues suitable for gene isolation and real-time PCR analysis
- Transgene inheritance in plants genetically engineered by microprojectile bombardment
- PCL-based composite scaffold matrices for tissue engineering applications
- Structural basis of matrix metalloproteinases and tissue inhibitors of metalloproteinases
- Application of chitosan-based nanocarriers in tumor-targeted drug delivery
- Electroporation-mediated gene transfer into intact nodal meristemsin planta
- Evanescent wave biosensors
- Transgenic grain legumes obtained byin planta electroporation-mediated gene transfer
- Neural networks as robust tools in drug lead discovery and development
- Methods for construction of adenovirus vectors
- Procedures for microencapsulation of enzymes, cells and genetically engineered microorganisms
- Cell adhesion molecules: potential therapeutic & diagnostic implications
- Resistance to macrolide, lincosamide, streptogramin, ketolide, and oxazolidinone antibiotics
- Knockdown of cellular RNA helicase DDX3 by short hairpin RNAs suppresses HIV-1 viral replication without inducing apoptosis
- Transient transfection factors for high-level recombinant protein production in suspension cultured mammalian cells
- On and around microtubules: an overview
- Analysis of HOX gene expression patterns in human breast cancer
- MMPs and TIMPs—an historical perspective
- Differential display
- A Pathogenic Role for Myelin-Specific Cd8+ T Cells in a Model for Multiple Sclerosis
- A novel chitosanase from Penicillium oxalicum M2 for chitooligosaccharide production: purification, identification and characterization
- Carbofuran-induced oxidative stress in mammalian brain
- Stable MSAP markers for the distinction of Vitis vinifera cv Pinot noir clones
- Cryopreservation of mammalian embryos
- Reference gene selection for real-time RT-PCR in eight kinds of rat regenerating hepatic cells
- The microRNA (miRNA): overview of the RNA genes that modulate gene function
- Magnaporthe oryzae populations adapted to finger millet and rice exhibit distinctive patterns of genetic diversity, sexuality and host interaction
- Heterologous expression of the chrysanthemum R2R3-MYB transcription factor CmMYB2 enhances drought and salinity tolerance, increases hypersensitivity to ABA …
- Properties of retinoids
- DNA fingerprinting techniques for microorganisms
- Safety and immunogenicity of a plant-produced recombinant monomer hemagglutinin-based influenza vaccine derived from influenza A (H1N1) pdm09 virus: a Phase …
- Musa genetic diversity revealed by SRAP and AFLP
- Strategies targeting telomerase inhibition
- Social networks and the entrepreneurial process in molecular biotechnology in Portugal: from science to industry
- Modifications of the histone N-terminal domains
- The role of redox mechanisms in cell signalling
- A simple method for the production of highly competent cells ofAgrobacterium for transformation via electroporation
- The important role of lipid peroxidation processes in aging and age dependent diseases
- Pyrosequence analysis of unamplified and whole genome amplified DNA from hydrocarbon-contaminated groundwater
- Delivery of DNA into mammalian cells by receptor-mediated endocytosis and gene therapy
- circlize implements and enhances circular visualization in R
- Degradative covalent reactions important to protein stability
- Decontamination of polymerase chain reaction reagents for detection of low concentrations of 16S rRNA genes
- G-quadruplexes: from guanine gels to chemotherapeutics
- Synonymous codon usage, GC3, and evolutionary patterns across plastomes of three pooid model species: emerging grass genome models for monocots
- Chiral separation principles in chromatographic and electromigration techniques
- Methods for general and molecular bacteriology
- Hybridization of DNA targets to glass-tethered oligonucleotide probes
- Purification, characterization of GH11 endo-β-1, 4-xylanase from thermotolerant Streptomyces sp. SWU10 and overexpression in Pichia pastoris KM71H
- On the effect of transient expression of mutated elF2α and elF4E eukaryotic translation initiation factors on reporter gene expression in mammalian cells upon cold …
- Optimal nitrogen supply as a key to increased and sustained production of a monoclonal full‐size antibody in BY‐2 suspension culture
- Design and implementation of high throughput screening assays
- Indian mustard aquaporin improves drought and heavy-metal resistance in tobacco
- Predictive models for transient protein expression in tobacco (Nicotiana tabacum L.) can optimize process time, yield, and downstream costs
- Pharmacogenetics of membrane transporters: an update on current approaches
- Pulsed-field gel electrophoresis
- An efficient random mutagenesis technique using anE. coli mutator strain
- Impacts of magnetic immobilization on the growth and metabolic status of recombinant Pichia pastoris
- Generation of recombinant antibodies
- Ribozyme as an approach for growth suppression of human pancreatic cancer
- Potential of molecular markers in plant biotechnology
- In vivo versus in vitro screening or selection for catalytic activity in enzymes and abzymes
- One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin
- Cationic β-cyclodextrin–chitosan conjugates as potential carrier for pmCherry-C1 gene delivery
- Improvement of Soybean; A Way Forward Transition from Genetic Engineering to New Plant Breeding Technologies
- Molecular biology and biotechnology: a comprehensive desk reference
- Site-directed mutagenesis by double polymerase chain reaction
- AAV as a viral vector for human gene therapy
- Quantitative analysis of gene expression by reverse transcription polymerase chain reaction and capillary electrophoresis with laser-induced fluorescence detection
- Nanoparticles, proteins, and nucleic acids: biotechnology meets materials science
- DNA chip analysis in diverse organisms with unsequenced genomes
- Apoptotic induction with bifunctional E. coli cytosine deaminase-uracil phosphoribosyltransferase mediated suicide gene therapy is synergized by curcumin treatment …
- Quantitative PCR
- Immobilization of bioactive protein A from Staphylococcus aureus (SpA) on the surface of Bacillus subtilis spores
- Molecular biotechnology for nutraceutical enrichment of food crops: the case of minerals and vitamins
- Private science: Biotechnology and the rise of the molecular sciences
- Biotechnology fundamentals
- Insertion of modifications in the β-globin locus using GET recombination with single-stranded oligonucleotides and denatured PCR fragments
- Polybrene/DMSO-assisted gene transfer
- Gene transfer into eukaryotic cells using activated polyamidoamine dendrimers
- Host‐induced gene silencing of an essential chitin synthase gene confers durable resistance to F usarium head blight and seedling blight in wheat
- Mycotechnology: the role of fungi in biotechnology
- Reverse transcriptase
- Biotechnology of microbial xylanases: enzymology, molecular biology, and application
- Molecular biology of spider silk
- PolyPhred: automating the detection and genotyping of single nucleotide substitutions using fluorescence-based resequencing
- Calculations for molecular biology and biotechnology
- Gene delivery efficiency in bone marrow-derived dendritic cells: comparison of four methods and optimization for lentivirus transduction
- RNA extraction from virus-diseased sweet potato for reverse transcriptase polymerase chain reaction analysis
- NUCEL (Cell and Molecular Therapy Center): a multidisciplinary center for translational research in Brazil
- Recombinant anti‐hCG antibodies retained in the endoplasmic reticulum of transformed plants lack core‐xylose and core‐α (1, 3)‐fucose residues
- Marine biotechnology advances towards applications in new functional foods
- The emerging technique of molecular imprinting and its future impact on biotechnology
- Production and Conjugation of Truncated Recombinant Diphtheria Toxin to VEGFR-2 Specific Nanobody and Evaluation of its Cytotoxic Effect on PC-3 Cell Line
- The application of molecular techniques in environmental biotechnology for monitoring
- Universal primers for the PCR amplification of dicot-lnfecting geminiviruses
- The unfolded protein response
- Recent advances of metabolomics in plant biotechnology
- Mycorrhiza: structure, function, molecular biology and biotechnology
- Blue Biotechnology: From Gene to Bioactive Product
- Probiotics and their fermented food products are beneficial for health
- Dendritic polyglycerol: a new versatile biocompatible material
- Illuminating the gateway of gene silencing: perspective of RNA interference technology in clinical therapeutics
- Biotechnology and pollution monitoring: use of molecular biomarkers in the aquatic environment
- Towards molecular farming in the future: transient protein expression in plants
- Chemically modified nucleic acid aptamers for in vitro selections: evolving evolution
- Molecular shuttles based on motor proteins: active transport in synthetic environments
- Brassica biotechnology: progress in cellular and molecular biology
- Towards molecular farming in the future: moving from diagnostic protein and antibody production in microbes to plants
- Endothelial progenitor cell–derived microvesicles activate an angiogenic program in endothelial cells by a horizontal transfer of mRNA
- Aptamers as therapeutic and diagnostic agents
- Applications of quantitative PCR in the biosafety and genetic stability assessment of biotechnology products
- Luminescent quantum dots for multiplexed biological detection and imaging
- Towards molecular farming in the future: Pichia pastoris‐based production of single‐chain antibody fragments
- Molecular markers in plant genetics and biotechnology
- Towards molecular farming in the future: using plant‐cell‐suspension cultures as bioreactors
- α-L-Arabinofuranosidases: biochemistry, molecular biology and application in biotechnology
- Annual plant reviews, functions and biotechnology of plant secondary metabolites
- Strength and structure of spiders’ silks
- New silk protein: modification of silk protein by gene engineering for production of biomaterials
- Design and synthesis of glycodendrimers
- Review on the development of genotyping methods for assessing farm animal diversity
- In vitro selection of nucleic acids for diagnostic applications
- Neoliberalism on the molecular scale. Economic and genetic reductionism in biotechnology battles
- Microvesicles derived from human adult mesenchymal stem cells protect against ischaemia–reperfusion-induced acute and chronic kidney injury
- Symbiosio of biotechnology and biomaterials: Applications in tissue engineering of bone and cartilage
- Dendrimers in medicine and biotechnology: new molecular tools
- ‘Anticalins’: a new class of engineered ligand-binding proteins with antibody-like properties
- Methods in plant molecular biology and biotechnology
- Molecular biology and biotechnology of flavonoid biosynthesis.
- Microbial production of spider silk proteins
- Biotechnology of plant osmotic stress tolerance physiological and molecular considerations
- Peptide nucleic acids on microarrays and other biosensors
- Environmental biotechnology
- Global plant-responding mechanisms to salt stress: physiological and molecular levels and implications in biotechnology
- Making dollars out of DNA: the first major patent in biotechnology and the commercialization of molecular biology, 1974-1980
- Identification of Potential Molecular Determinants of Murine Embryonic Stem Cell Differentiation by a Transposon-Based Approach
- The systems biology markup language (SBML): a medium for representation and exchange of biochemical network models
- Glycodendrimers: novel glycotope isosteres unmasking sugar coding. Case study with T-antigen markers from breast cancer MUC1 glycoprotein
- Squalene–biochemistry, molecular biology, process biotechnology, and applications
- Virtual screening SNP-polymorphisms of genes determining the high level of general non-specific reactivity of organism
- Design, functional evaluation and biomedical applications of carbohydrate dendrimers (glycodendrimers)
- Research and biotechnology in sea buckthorn (Hippophae spp.)
- Genetic evidence for a predominant role of PI3Kβ catalytic activity in ITAM-and integrin-mediated signaling in platelets
- Time resolved amplification of cryptate emission: a versatile technology to trace biomolecular interactions
- Chemicals from biotechnology: molecular plant genetics will challenge the chemical and the fermentation industry
- Inteins, valuable genetic elements in molecular biology and biotechnology
- Stem-loop oligonucleotides: a robust tool for molecular biology and biotechnology
- Lutetium labeled-siRNA Delivery into Colon Cancer Cells Using Liposomes: A Comparative Study
- A critical review of the concept of transgenic plants: insights into pharmaceutical biotechnology and molecular farming
- Design and characterization of molecular tools for a synthetic biology approach towards developing cyanobacterial biotechnology
- Quenched probes for highly specific detection of cellular RNAs
- Mechanisms of protein folding: molecular chaperones and their application in biotechnology
- Modified Nucleosides: In Biochemistry, Biotechnology and Medicine
- Surface engineering of a cutinase from Thermobifida cellulosilytica for improved polyester hydrolysis
- Synthesis of fluorescent metallic nanoclusters toward biomedical application: recent progress and present challenges
- Acyl-CoA: diacylglycerol acyltransferase: molecular biology, biochemistry and biotechnology
- Application and current trends of biotechnology: a brief review
- Molecular regulation of cell‐cycle progression and apoptosis in mammalian cells: implications for biotechnology
- Candida rugosa lipases: Molecular biology and versatility in biotechnology
- … of next generation sequencing and junction sequence analysis bioinformatics to achieve molecular characterization of crops improved through modern biotechnology
- Research on the effects of the ‘Dance of the Spiral’methodology upon the physiological parameters of plants and the essential oil content.