Moleculer Weight Determination in Moleculer Spectroscopy

Moleculer Weight Determination in Moleculer Spectroscopy

Moleculer epectroscopy is an important instrument used for identifying a variety of molecular characteristic:

  • including their structure
  • chemical make-up
  • and molecular weight

Molecular weight is a important factor that gives information about a molecule’s size.

Molecular weight could be calculated by following moleculer spectroscopic method:

Mass Spectrometry:

This exceptional analytical method can be used to explain the molecular weight of a substance. This method involves ionising the material and arranging the ions according to their mass-to-charge ratios.

Gel Permeation Chromatography:

Using a technology called gel permeation chromatography (GPC), molecules are separated according to their sizes. In GPC, a column containing a stationary phase of porous beads is used to pass the sample through. While the smaller molecules can find their way across the pores and are thus separated from the larger molecules, the larger molecules are remains at the boundry of the pores. The sample’s molecular weight can be determined from the elution profile.

Light Scattering:

Measurement of the scattering of light by particles in a solution is done using a technique called light scattering.  Intensity of the light depend upon the size of the particles . The sample’s molecular weight can be calculted by examining the scattering pattern.

Note:

You have to choose technique according to available material and demanding level of precision for molecular weight determination.