UVI Visible Spectroscopy

UVI Visible Spectroscopy in forensic chemistry

It is a technique which is used for determining how much light is absorbed in the visible and ultraviolet  portions(approximately 380-800nm) of the electromagnetic spectrum and is known as UV-visible spectroscopy.

 Uses of UV- VISIBLE spectroscopy

It is often used to

  • identify and classify chemical compounds
  • determine the composition of

A beam of light is transmitted across a sample in UV-visible spectroscopy, and the amount of light absorbed  or trapped by the sample is then measured. This absorbed light is provide energy to  sample’s electrons which then excited to higher energy levels. The concentration of the absorbing species in the sample directly related to  how much absorption occurs.


Applications for UV-visible spectroscopy include varioue field such as in

  • analytical chemistry
  • biology
  • and in environmental science.


Chromophores are chemical substances that absorb light in the UV-visible range, and their concentration is frequently examined using this technique. Examples of chromophores include

  • aromatic compounds
  • conjugated double bonds
  • and functional groups like carbonyl and amino groups

The electrical structure of molecules can also be studied using UV-visible spectroscopy, and functional groups can be recognized based on their patterns of absorption. It is a valuble  technique as it is used for studying complicated combinations of substances where it can identify various chromophores and present data according to their amounts.

In general, Uv visible  is a flexible technique and it also used  analytical method that is significant in many fields of research and technology.