Basic Elements Of Practical Spectroscopy
The method of spectroscopy is a broad field deal with the study of interaction of light with matter .It is widely used in various discipline such as In
- Chemistry
- physics
- biology and astronomy.
Practical spectroscopy’s foundational components are:
Radiation source:
Radiation are required in order to provide energy to the sample which is used to generate the spectral signal.
Different sources may be used such as
- A lightbulb
- Laser
- or synchrotron.
Sample holder:
The sample holder is used for holding of sample while performing experiment. To eradicate any spectrum ambuigity, the must be transparent to the radiation.
Detector:
After the radiation has gone through the sample, a detector is utilised to gauge its intensity.
Different detector may be used according to the technique of eprctroscopy
- The detector may be a photomultiplier tube
- charge-coupled device (CCD)
- or a Fourier transform infrared (FTIR) detector.
Monochromater:
Incoming radiation is separated into its constituent wavelengths using a monochromator. Using a prism or diffraction grating allows for this.
Optical filters:
The employment of optical filters allows for the selective transmission of some light wavelengths while blocking others.
Uses:
Opticle fiber is applicable to
- enhancing the signal-to-noise ratio
- and filter out undesired background noise.
Calibration standards:
To check the equipment and for getting information about the precision of the measurements, calibration standards are needed. Reference materials with well-known spectrum characteristics could be included in these standards.
Software for data analysis:
To process and analyse the spectral data, software is used. Software for peak fitting, spectral matching, and spectral deconvolution may be included in this.
