Adsorption chromatography
Adsorption chromatography is a form of chromatography in which a solid material is form a stationery phase with a large surface area, such as silica gel or alumina, that adsorbs the solutes from the mobile phase. This technique separates the components of a mixture based on their different affinities for the stationary phase.
How it work?
- The sample is put on to the top of the stationary phase, which is usually packed into a column.
- The mobile phase, usually a solvent, is then passed through the column, allowing the different components to interact with the stationary phase and separate based on their affinities.
Examples of adsorption chromatography
Some of the examples of adsorption chromatography are given below:
- Silica gel chromatography
- Alumina chromatography
- Ion exchange chromatography
- Size exclusion chromatograph
- Hydrophobic interaction chromatography
- Affinity chromatography
- Chiral chromatography
Silica gel chromatography:
This technique consumes silica gel as the stationary phase to segregate components founded on their resemblance for the stationary phase.
Alumina chromatography:
Simmilar to silica gel chromatography, alumina act as a stationary phase in this technique.
Ion exchange chromatography:
in this technique, the stationary phase comprises of charged groups that attract and segregate charged analytes from the sample.
Size exclusion chromatography:
This technique uses molecular size or weight to segregate the sample with larger molecules eluting first.
Hydrophobic interaction chromatography:
Stationary phase used in this technique is a hydrophobic material that react with hydrophobic regions of the sample, leading to segregation founded on hydrophobicity.
Affinity chromatography:
Affinity chromatography uses a stationary phase that particularly attach to a target molecule, letting for selective purification or segregation of the target.
Chiral chromatography:
Chiral chromatography segregate enantiomers (mirror-image isomers) of a compound utilizing a chiral stationary phase.
NOTE:
These are some examples of the many types of adsorption chromatography that are normally utilized in
- scientific research
- pharmaceutical development
- and industrial processes.