Which of the following is not a restriction enzyme?

By: Prof. Dr. Fazal Rehman | Last updated: February 3, 2024

 

QUESTION: Which of the following is not a restriction enzyme?

A    Eco R1

B    Chitinase

C    Bam H1

D    Hind -II

ANSWER:    Chitinase

 

Name of restriction enzyme Full form Recognition site sequence                     Uses
EcoRI Escherichia coli RY13 GAATTC  

·        It generates sticky ends thus used in constructing recombinant DNA molecules.

·        Cleaves DNA at specific recognition sites, enabling analysis of DNA fragments.

·        Used to create DNA fragments with known sizes for mapping the positions of restriction sites.

Bam HI Bacillus amyloliquefaciens H GGATCC  

·        It produces sticky ends that can be ligated into compatible vectors, enabling gene cloning.

·        It cuts DNA at specific sites, helping in the analysis of DNA fragments.

HindIII

 

 

 

Haemophilus influenzae Rd

 

 

AAGCTT

 

 

 

 

·        Cleaves DNA at specific recognition sites, often used to generate smaller fragments for analysis or manipulation.

·        DNA Analysis: Produces distinct fragments, allowing for DNA fingerprinting and size determination.

·        Fragments generated can be separated by gel electrophoresis for various applications.

XhoI Xanthomonas homogena CTCGAG  

·        Produces compatible ends for ligation with vectors, commonly used in recombinant DNA technology.

EcoRV Escherichia coli RV GATATC  

·        Generates DNA fragments for mapping and analyzing restriction sites on DNA molecules.

NotI Nocardia otitidis GCGGCCGC  

·        Produces DNA fragments with defined ends thus facilities in precise mapping of DNA sequences.

SacI Streptomyces achromogenes GAGCTC  

·        Used to insert DNA fragments into expression vectors for studying gene function.

KpnI Klebsiella pneumoniae GGTACC  

·        It produces cohesive ends for ligation with compatible DNA fragments or vectors.

PstI Providencia stuartii CTGCAG ·        It is often used in the digestion of DNA and for generating sticky ends.
SalI Streptomyces albus G GTCGAC  

·        This enzyme cuts DNA at specific sites, facilitating gene cloning and recombinant DNA construction.

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