QUESTION: Which of the following is not a restriction enzyme?
A Eco R1
B Chitinase
C Bam H1
D Hind -II
ANSWER: Chitinase
Name of restriction enzyme | Full form | Recognition site sequence | Uses |
EcoRI | Escherichia coli RY13 | GAATTC |
· It generates sticky ends thus used in constructing recombinant DNA molecules. · Cleaves DNA at specific recognition sites, enabling analysis of DNA fragments. · Used to create DNA fragments with known sizes for mapping the positions of restriction sites. |
Bam HI | Bacillus amyloliquefaciens H | GGATCC |
· It produces sticky ends that can be ligated into compatible vectors, enabling gene cloning. · It cuts DNA at specific sites, helping in the analysis of DNA fragments. |
HindIII
|
Haemophilus influenzae Rd
|
AAGCTT
|
· Cleaves DNA at specific recognition sites, often used to generate smaller fragments for analysis or manipulation. · DNA Analysis: Produces distinct fragments, allowing for DNA fingerprinting and size determination. · Fragments generated can be separated by gel electrophoresis for various applications. |
XhoI | Xanthomonas homogena | CTCGAG |
· Produces compatible ends for ligation with vectors, commonly used in recombinant DNA technology. |
EcoRV | Escherichia coli RV | GATATC |
· Generates DNA fragments for mapping and analyzing restriction sites on DNA molecules. |
NotI | Nocardia otitidis | GCGGCCGC |
· Produces DNA fragments with defined ends thus facilities in precise mapping of DNA sequences. |
SacI | Streptomyces achromogenes | GAGCTC |
· Used to insert DNA fragments into expression vectors for studying gene function. |
KpnI | Klebsiella pneumoniae | GGTACC |
· It produces cohesive ends for ligation with compatible DNA fragments or vectors. |
PstI | Providencia stuartii | CTGCAG | · It is often used in the digestion of DNA and for generating sticky ends. |
SalI | Streptomyces albus G | GTCGAC |
· This enzyme cuts DNA at specific sites, facilitating gene cloning and recombinant DNA construction. |