Guess Paper 1: Genetic Engineering Fall – 2020 Past Papers
Time Allowed: 3 hours
Total Marks: 70, Passing Marks (35)
Q.1. A. Fill in the blanks with appropriate words. (7)
i. Restriction enzymes capable of making internal cuts in a DNA molecule is called………..
ii. Bacterium which is able to synthesize human growth hormone is known as………………….
iii. Enzyme used to join DNA fragment is called……………
iv. PCR was invented by…………..
v. Library screening identifies colonies which have specific…………….
vi. Vectors commonly used for sequencing human genome …………..
vii. The natural function of restriction enzyme is …….…
B. Select the most appropriate answer from the choices given. (7)
i. Which of the following ions are required for the activity of Type II restriction enzyme…….
a. Ca2+ b. Mg2+ c. Cl2+ d. Mn2+
ii. Telomeric sequences are found in…….. ……..
a. HAC b. BAC c. YAC d. PAC
iii. Taq polymerase is used in PCR because of it’s……….
a. Low thermal stability b. High fidelity c. High speed d. High thermal stability
iv. The gene formed by the joining of the DNA segments from two different sources
are called as……………..
a. Recombinant Gene b. Joined gene c. Chimeric gene d. Both A and B
v. Genomic library is normally made by………….
a. α phage vector b. λ phage vector c. β phage vector d. γ phage vector
vi. Long probes are usually made by ……………….
a. Gene expression b. Cloning c. Hybridization d. All of the above
vii. Which of the following is suitable for identifying DNA molecule in a sample……?
a. Western blot b. Southern blot c. Northern blot d. Eastern blot
Q.2. i. What are restriction enzymes and how they recognize specific sequences? (4)
ii. Explain expression system for prokaryotes and eukaryotes. (5)
iii. Advantages and disadvantages of genetic engineering. (5)
Q.3. Explain the construction and uses of the following cloning vehicles.
i. Plasmids ii. Cosmids iii. Phagemids iv. YAC v.BAC vi. HAC vii. PBR322 (14)
Q.4. i. Describe different methods for screening the clones. (7)
ii. Explain the mechanism behind the exchange of genes between a lambda phage and Col EI factor.
(7)
Q.5. Describe in detail different methods of introducing exogenous DNA into cell. (14)
Q.6. i. What is PCR? Describe its uses and principle in detail. (7)
ii. Explain different methods used for DNA sequencing. (7)
Q.7 i. Describe differences between primer and probe. (4)
ii. Explain restriction modification system. (5)
iii. What are the different principles of nucleic acid isolation? (5)
Q.8. Write short notes on the following
i. Slab gel electrophoresis (5)
ii. Scope of genetic engineering (5)
iii. PCR types (4)
