Which of the following is the first step of glycolysis?

Question: Which of the following is the first step of glycolysis?

A Breakdown of glucose

B Phosphorylation of glucose

C Conversion of glucose to fructose

D  Dehydrogenation of glucose

Answer:  Phosphorylation of glucose

           Introduction Steps of glycolysis Enzyme involved


·        Glycolysis occurs in the cytoplasm of the cell, generating ATP through substrate-level phosphorylation.

·        It is also known as E.M.P. pathway which stands for Embden – Meyerhof – Parnas Pathway.

·        It was discovered by Gustav Embden, Otto Meyerhof, and Jakub Karol Parnas


Step 1: Phosphorylation of Glucose Hexokinase
Step 2: Isomerization Glucose-6-Phosphate Phosphoglucose isomerase

Step 3: Second Phosphorylation


Step 4: Cleavage of Fructose 1,6-Bisphosphate


Step 5: Isomerization of Dihydroxyacetone Phosphate


Triose phosphate isomerase

Step 7: Substrate-Level Phosphorylation

Phosphoglycerate kinase

Step 8:  Inter-Molecular Shift of Phosphate Group


Phosphoglycerate mutase

Step 9: Dehydration




Step 10: Conversion of Phosphoenolpyruvate to Pyruvate


Pyruvate kinase