Past Papers Clinical Microbiology

By: Prof. Fazal Rehman Shamil

Guess Paper 1: Clinical Microbiology Fall – 2020 Past Papers

University Name – Confidential

Time Allowed: 3 hours

Total Marks:    70, Passing Marks (35)

Q. No. 1: Mark the following statements either as True or False.(10 marks)
a) All the staphylococci can ferment mannitol(True / False)
b) CSF is a sterile medium (True / False)
c) If acids get on your skin or clothes, wash at once with oil (True / False)
d) Tuberculosis can affect bones (True / False)
e) Specimen collected only at the disease site (True / False)
f) Salmonella can ferment lactose(True / False)
g) Streptococcus pyogens causes bloody diarrhea(True / False)
h) Blood samples initially collected will detect 99 percent of bloodstream infections within 24 hours (True / False)
i) pH and volume of urine does not matter during (True / False)
j) Staphylococcus lack the ability to degrade gelatin in the medium (True / False)
Q. No. 2: Explain the common pathogens found in blood. Write a detail overview of their identification(15 marks)

Q. No. 3: How wounds swabs are examined? What are common pathogenic organism that are identified(15)

Q. No. 4: Write the clinical features of tuberculosis. How tuberculosis can be diagnosed in the laboratory(15)

Q. No. 5: What are the lab safety plans for Microbiology lab(15 marks)

Q. No. 6: Explain the common pathogens found in blood. Write a detail overview of their identification (15)

Q. No. 7: Explain the collection and analysis of urine specimens in clinical microbiology laboratory (15)

Guess Paper 2: Clinical Microbiology Spring – 2020 Past Papers

University Name – Confidential

Time Allowed: 3 hours

Total Marks:    70, Passing Marks (35)

Q No. 1: Mark the following statement either as True or False. (14 Marks)
1. Engineering controls, personal protective equipment, hygiene practices, and administrative controls each play a role in a comprehensive laboratory safety program. (T/F)
2. Fume Hood is designed to capture chemicals that escape from their containers or apparatus and to remove them from the laboratory environment before they can be inhaled. (T/F)
3. Biological Safety Cabinets (BSCs), are the primary means of containment for working safely with infectious microorganisms. (T/F)
4. ‘High hazard’ research is that which due to the nature of the hazard, or the quantity of the material, or the potential for exposure poses higher than usual risk to the worker. (T/F)
5. Anaerobic bacteria are common causes of infection and will be missed in clinical diagnosis unless special precautions are taken for their isolation and culture. (T/F)
6. Infections of the bloodstream are most commonly caused by bacteria (bacteremia) but can also be caused by yeasts or other fungi . (T/F)

7. An increased white blood cell (WBC) count (or in some cases a decreased WBC count) may indicate infection. (T/F)

8. Escherichia coli Klebsiella strains Other enterobacteria Pseudomonas aeruginosa Bacteroides species are the common pathogen of ASCITIC FLUID. (T/F)
9. A transudate contains Less than 100/ mm3 cells, (T/F)

10. Exudate usually contains more than1000/ mm3 cells. (T/F)

11. A transudate in ASCITIC FLUID usually contains less than 2.5 g/dl o protein whereas an exudate contains more than 3g/dl. (T/F)

12. The introduction of nucleic acid amplification tests (NAAT) for the diagnosis of genital Chlamydia trachomatis infection was a major step forward for laboratory-based studies on this important pathogen (T/F)
13. During the pelvic exam, the health care provider takes swabs from the cervix and/or vagina of man to test for chlamydia and gonorrhea (T/F)

14. HVS is a technique used in Obstetrics and Gynaecology to obtain a sample of discharge from the vagina. (T/F)

Part B (56 Marks)

Q.2 How different body effusion are collected, transported and examined? (14)

Q.3 Write down traditional, Culture, and Molecular method of TB Diagnosis? (14)

Q.4 How vaginal and cervical swabs of females and urethral discharge of male should be transported culture and what parameter should be considered while diagnosis a disease in respect to these specimens. (14)

Q.5 Is a wound swab for microbiological analysis supportive in the clinical assessment of infection of a chronic wound and how would you proceed a swab for culture & susceptibility (C & S) in Suspected Wound Infection? (14)

Q.6 (a) How Capnophiles bacteria should be isolated and what are the specific growth media and parameter for Capnophiles? (7)
(b) Write a details note on anaerobic culture techniques? (7)

Q.7 Write down the general guidelines in details regarding specimen handling (14)

Guess Paper 3: Clinical Microbiology Fall – 2019 Past Papers

University Name – Confidential

Time Allowed: 3 hours

Total Marks:    70, Passing Marks (35)

Q No. 1: Mark the following statement either as True or False. (14 Marks)
1. Engineering controls, personal protective equipment, hygiene practices, and administrative controls each play a role in a comprehensive laboratory safety program. (T/F)
2. Fume Hood is designed to capture chemicals that escape from their containers or apparatus and to remove them from the laboratory environment before they can be inhaled. (T/F)
3. Biological Safety Cabinets (BSCs), are the primary means of containment for working safely with infectious microorganisms. (T/F)
4. ‘High hazard’ research is that which due to the nature of the hazard, or the quantity of the material, or the potential for exposure poses higher than usual risk to the worker. (T/F)
5. Anaerobic bacteria are common causes of infection and will be missed in clinical diagnosis unless special precautions are taken for their isolation and culture. (T/F)
6. Infections of the bloodstream are most commonly caused by bacteria (bacteremia) but can also be caused by yeasts or other fungi . (T/F)

7. An increased white blood cell (WBC) count (or in some cases a decreased WBC count) may indicate infection. (T/F)

8. Escherichia coli Klebsiella strains Other enterobacteria Pseudomonas aeruginosa Bacteroides species are the common pathogen of ASCITIC FLUID. (T/F)
9. A transudate contains Less than 100/ mm3 cells, (T/F)

10. Exudate usually contains more than1000/ mm3 cells. (T/F)

11. A transudate in ASCITIC FLUID usually contains less than 2.5 g/dl o protein whereas an exudate contains more than 3g/dl. (T/F)

12. The introduction of nucleic acid amplification tests (NAAT) for the diagnosis of genital Chlamydia trachomatis infection was a major step forward for laboratory-based studies on this important pathogen (T/F)
13. During the pelvic exam, the health care provider takes swabs from the cervix and/or vagina of man to test for chlamydia and gonorrhea (T/F)

14. HVS is a technique used in Obstetrics and Gynaecology to obtain a sample of discharge from the vagina. (T/F)

Part B (56 Marks)

Q.2 How different body effusion are collected, transported and examined? (14)

Q.3 Write down traditional, Culture, and Molecular method of TB Diagnosis? (14)

Q.4 How vaginal and cervical swabs of females and urethral discharge of male should be transported culture and what parameter should be considered while diagnosis disease in respect to these specimens. (14)

Q.5 Is a wound swab for microbiological analysis supportive in the clinical assessment of infection of a chronic wound and how would you proceed a swab for culture & susceptibility (C & S) in Suspected Wound Infection? (14)

Q.6 (a) How Capnophiles bacteria should be isolated and what are the specific growth media and parameter for Capnophiles? (7)
(b) Write a details note on anaerobic culture techniques? (7)

Q.7 Write down the general guidelines in details regarding specimen handling (14)

Prof. Fazal Rehman Shamil