Diagnostic Microbiology MCQs

By: Prof. Dr. Fazal Rehman | Last updated: July 27, 2024

  1. Which staining technique is commonly used to identify Mycobacterium tuberculosis in clinical specimens?
    a) Gram staining
    b) Acid-fast staining
    c) Giemsa staining
    d) Silver stainingAnswer: b) Acid-fast staining
  2. What is the primary culture medium used to isolate and differentiate Gram-negative bacteria in clinical microbiology?
    a) Blood agar
    b) MacConkey agar
    c) Sabouraud agar
    d) Nutrient agarAnswer: b) MacConkey agar
  3. Which test is used to determine the antibiotic susceptibility of bacteria?
    a) Gram staining
    b) Kirby-Bauer disk diffusion test
    c) Coagulase test
    d) Catalase testAnswer: b) Kirby-Bauer disk diffusion test
  4. Which type of medium is used to cultivate fungi from clinical samples?
    a) Chocolate agar
    b) Cysteine-lactose-electrolyte-deficient (CLED) agar
    c) Sabouraud dextrose agar
    d) Thayer-Martin agarAnswer: c) Sabouraud dextrose agar
  5. In microbiology, what does a positive catalase test indicate?
    a) The presence of coagulase
    b) The presence of catalase enzyme
    c) The presence of oxidase enzyme
    d) The presence of urease enzymeAnswer: b) The presence of catalase enzyme
  6. Which bacterium is commonly identified using the urease test?
    a) Escherichia coli
    b) Staphylococcus aureus
    c) Helicobacter pylori
    d) Neisseria gonorrhoeaeAnswer: c) Helicobacter pylori
  7. What type of bacteria can be differentiated using the blood agar hemolysis test?
    a) Gram-positive cocci
    b) Gram-negative bacilli
    c) Anaerobic bacteria
    d) MycobacteriaAnswer: a) Gram-positive cocci
  8. Which test is used to confirm the presence of Staphylococcus aureus?
    a) Coagulase test
    b) Oxidase test
    c) Indole test
    d) Methyl red testAnswer: a) Coagulase test
  9. Which medium is used to isolate and identify Pseudomonas aeruginosa?
    a) Blood agar
    b) MacConkey agar
    c) Cetrimide agar
    d) Eosin methylene blue (EMB) agarAnswer: c) Cetrimide agar
  10. Which diagnostic method is used for identifying viral infections in clinical specimens?
    a) PCR (Polymerase Chain Reaction)
    b) Gram staining
    c) Blood agar culture
    d) Catalase testAnswer: a) PCR (Polymerase Chain Reaction)
  11. What is the purpose of using the API test in microbiology?
    a) To identify bacterial species based on their metabolic activities
    b) To test antibiotic susceptibility
    c) To determine bacterial morphology
    d) To isolate fungi from clinical samplesAnswer: a) To identify bacterial species based on their metabolic activities
  12. Which of the following is a selective medium for the isolation of Salmonella species?
    a) Xylose lysine deoxycholate (XLD) agar
    b) Nutrient agar
    c) Sabouraud agar
    d) Blood agarAnswer: a) Xylose lysine deoxycholate (XLD) agar
  13. Which test is used to identify bacteria that produce hydrogen sulfide (H2S)?
    a) Sulfide-indole-motility (SIM) test
    b) Urease test
    c) Catalase test
    d) Coagulase testAnswer: a) Sulfide-indole-motility (SIM) test
  14. What type of agar is used to differentiate between Staphylococcus epidermidis and Staphylococcus aureus?
    a) Mannitol salt agar
    b) Blood agar
    c) MacConkey agar
    d) Sabouraud dextrose agarAnswer: a) Mannitol salt agar
  15. Which of the following tests is used to detect the production of acid from carbohydrates by bacteria?
    a) Voges-Proskauer test
    b) Methyl red test
    c) Phenol red carbohydrate fermentation test
    d) Indole testAnswer: c) Phenol red carbohydrate fermentation test

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