1. In microbiology, what does a positive catalase test indicate?
(A) The presence of coagulase
(B) The presence of urease enzyme
(C) The presence of oxidase enzyme
(D) The presence of catalase enzyme
2. What is the primary culture medium used to isolate and differentiate Gram-negative bacteria in clinical microbiology?
(A) Blood agar
(B) Nutrient agar
(C) Sabouraud agar
(D) MacConkey agar
3. What is the purpose of using the API test in microbiology?
(A) To test antibiotic susceptibility
(B) To identify bacterial species based on their metabolic activities
(C) To determine bacterial morphology
(D) To isolate fungi from clinical samples
4. What type of agar is used to differentiate between Staphylococcus epidermidis and Staphylococcus aureus?
(A) Blood agar
(B) Mannitol salt agar
(C) MacConkey agar
(D) Sabouraud dextrose agar
5. What type of bacteria can be differentiated using the blood agar hemolysis test?
(A) Mycobacteria
(B) Gram-negative bacilli
(C) Anaerobic bacteria
(D) Gram-positive cocci
6. Which bacterium is commonly identified using the urease test?
(A) Escherichia coli
(B) Staphylococcus aureus
(C) Neisseria gonorrhoeae
(D) Helicobacter pylori
7. Which diagnostic method is used for identifying viral infections in clinical specimens?
(A) PCR (Polymerase Chain Reaction)
(B) Gram staining
(C) Blood agar culture
(D) Catalase test
8. Which medium is used to isolate and identify Pseudomonas aeruginosa?
(A) Blood agar
(B) MacConkey agar
(C) Cetrimide agar
(D) Eosin methylene blue (EMB) agar
9. Which of the following is a selective medium for the isolation of Salmonella species?
(A) Sabouraud agar
(B) Nutrient agar
(C) Xylose lysine deoxycholate (XLD) agar
(D) Blood agar
10. Which of the following tests is used to detect the production of acid from carbohydrates by bacteria?
(A) Phenol red carbohydrate fermentation test
(B) Methyl red test
(C) Voges-Proskauer test
(D) Indole test
11. Which staining technique is commonly used to identify Mycobacterium tuberculosis in clinical specimens?
(A) Acid-fast staining
(B) Gram staining
(C) Giemsa staining
(D) Silver staining
12. Which test is used to confirm the presence of Staphylococcus aureus?
(A) Coagulase test
(B) Oxidase test
(C) Indole test
(D) Methyl red test
13. Which test is used to determine the antibiotic susceptibility of bacteria?
(A) Gram staining
(B) Coagulase test
(C) Kirby-Bauer disk diffusion test
(D) Catalase test
14. Which test is used to identify bacteria that produce hydrogen sulfide (H2S)?
(A) Catalase test
(B) Urease test
(C) Sulfide-indole-motility (SIM) test
(D) Coagulase test
15. Which type of medium is used to cultivate fungi from clinical samples?
(A) Chocolate agar
(B) Cysteine-lactose-electrolyte-deficient (CLED) agar
(C) Thayer-Martin agar
(D) Sabouraud dextrose agar