[OBJECTIVE]
Subject: Biodegradation and Bioremediation
Time Allowed: 15 Min
Total Marks: 10
NOTE: ATTEMPT THIS PAPER ON THIS QUESTION SHEET ONLY. Division of marks is given in front of each question. This Paper will be collected back after expiry of time limit mentioned above.
Part-I Encircle the correct option, each question carries equal marks. (10)
1. The use of microorganisms to degrade environmental pollutants is known as:
A. Bio stimulation
B. Bioremediation
C. Biotransformation
D. All of these
2. Microorganisms remove metals by
A. Adsorption
B. Formation of new compounds
C. Complexion
D. All of these
3. Which cleanup approach involves removing groundwater or soil from its natural setting to allow Ibr bioremediation?
A) In situ bioremediation
B) Ex situ bioremediation
C) Bioaugmentation
D) Phytoremediation
4. Which of the following waste water treatments is most likely to produce carcinogens as a byproduct?
A. Chlorination
B. Ultraviolet light (UV)
C. Sand filtration
D. Carbon filtration
5. The enzyme nitrogenase is produced by it
A. Pathogenic bacteria
B. Anaerobic bacteria
C. Cyanobacteria
D. Both B&C
6. A major organism used in commercial bioleaching for copper recovery is:
A. Desulfovibrio desulfuricans
B. Pseudomonas aeruginosa
C. Aspergillus niger
D. Thiobacillus ferrooxidans
7. Organophosphate pesticides can be detoxified by which enzymes of microbes.
A. Reductase
B. Oxidases
C. Hydrolases
D. Oxygenases
8. Identification of the sequence of genes in a chromosome is known as:
A. Gene mapping
B. Karyotype
C. Gene coding
D. Gene linkage
9. Anaerobic bacteria often play important roles in bioremediation. Which of the following is not an electron acceptor used by anaerobes during biodegradation reactions?
CO2
NO3
Fe (III)
H2O
10. During which stage of wastewater treatment is the primary effluent aerated to allow for biodegradation by aerobic microbes?
A. Sedimentation
B. Secondary treatment
C. Sludge digestion
[SUBJECTIVE]
Subject: Biodegradation and Bioremediation
Time Allowed: 2 Hour 45 Min
Total Marks: 50
NOTE: ATTEMPT THIS (SUBJECTIVE) ON THE SEPARATE ANSWER SHEET PROVIDED
Part-II Give short answers, each question carries equal marks. (20)
Q#1: What do you mean by organotrophic bacteria?
Q#2: Write down the basic requirements for bacterial growth during Bioremediation?
Q#3: Describe the Process of Sorption and its Effects on Biodegradation.
Q#4: Describe the Role of Co-metabolism in Bioremediation
Q#5: What is ACTIVATION? Enlist mechanisms involve in ACTIVATION.
Q#6: What is meant by detoxification? Enlist mechanisms may involve in detoxification.
Q#7: Describe the process of sorption and its effect on biodegradation.
Q#8: Describe the Effects of Heavy Metals on Environment?
Q#9: What is meant by bioreactor-based method of bioremediation?
Q#10: What is detoxification? Enlist mechanisms may involve in detoxification.
Part-III Give detailed answers, each question carries equal marks. (30)
Q#1: (a) Write a note on anthropogenic impact on environmental pollution?
(b) What is the role of Genetic modification for bioremediation process?
Q#2: (a) Define Xenobiotics and their role in our environment.
(b) Write a note on biocatalyst selection for bioremediation.
Q#3: (a) Define Biotechnology; describe its role for the removal of pollutants.
(b) What are plasmid, explain the function of catabolic plasmid for Biodegradation
