Question: which enzyme adds new nucleotides?
Answer:
- DNA polymerase adds new nucleotides during DNA replication.
- It catalyzes the formation of phosphodiester bonds between the incoming nucleotide triphosphates and the growing DNA strand.
- There are several types of DNA polymerases involved in DNA replication, including
- DNA polymerase alpha (Pol α)
- DNA polymerase delta (Pol δ)
- DNA polymerase epsilon (Pol ε) in eukaryotes
- DNA polymerase III (Pol III) in prokaryotes
| Enzyme | Description | Function |
| DNA Polymerase | Enzyme involved in DNA replication | Adds new nucleotides to the growing DNA strand |
| Types | Various types exist, e.g., Pol α, δ, ε (eukaryotes) and Pol III (prokaryotes) | Carry out DNA replication and repair |
| Proofreading | Many DNA polymerases possess proofreading activity | Corrects errors by removing incorrect nucleotides |
| Exonuclease activity | Some DNA polymerases possess 3′ to 5′ exonuclease activity | Removes incorrect nucleotides during proofreading |
| Leading Strand | Synthesized continuously in the 5′ to 3′ direction by DNA polymerase | Requires a single primer for initiation |
| Lagging Strand | Synthesized discontinuously in the form of Okazaki fragments | Requires multiple primers for initiation |
Important Questions about Enzymes
- Enzymes MCQs
- Inhibition in enzymes
- Which enzyme digests starch?
- Which enzyme digests protein?
- which enzyme is present in saliva?
- Which enzyme is not present in succus entericus?
- Which enzyme breaks down protein in the stomach?
- Which enzyme converts fibrinogen to fibrin?
- Which enzyme converts glucose into alcohol?
- Which enzyme digests fat?
- Which enzyme digests milk protein?
- Which enzyme is used in PCR?
- Which enzyme unzips the DNA double helix?
- Which liver enzyme increases with alcohol?
- Which enzyme cuts DNA?
- Which enzyme catalyzes the first step of glycolysis?
- Which enzyme binds DNA fragments together?
- Which enzyme transcribes DNA into RNA?
- which enzyme adds new nucleotides?
- Which enzyme digests cellulose?
- Which enzyme separates the two strands of DNA?
- Which enzyme converts carbohydrates into glucose?
- Which enzyme removes RNA primer?
- Which enzymes help in digestion?
- Which enzyme works best in acidic conditions?
- Which enzyme synthesizes tRNA?
- Which enzyme secreted by pancreas?
- Which enzyme converts maltose into glucose?
- Which vitamins work as coenzyme?