QUESTION: Which of the following is not a restriction enzyme?
A Eco R1
B Chitinase
C Bam H1
D Hind -II
ANSWER: Chitinase
| Name of restriction enzyme | Full form | Recognition site sequence | Uses |
| EcoRI | Escherichia coli RY13 | GAATTC | · It generates sticky ends thus used in constructing recombinant DNA molecules. · Cleaves DNA at specific recognition sites, enabling analysis of DNA fragments. · Used to create DNA fragments with known sizes for mapping the positions of restriction sites. |
| Bam HI | Bacillus amyloliquefaciens H | GGATCC | · It produces sticky ends that can be ligated into compatible vectors, enabling gene cloning. · It cuts DNA at specific sites, helping in the analysis of DNA fragments. |
| HindIII
| Haemophilus influenzae Rd
| AAGCTT
| · Cleaves DNA at specific recognition sites, often used to generate smaller fragments for analysis or manipulation. · DNA Analysis: Produces distinct fragments, allowing for DNA fingerprinting and size determination. · Fragments generated can be separated by gel electrophoresis for various applications. |
| XhoI | Xanthomonas homogena | CTCGAG | · Produces compatible ends for ligation with vectors, commonly used in recombinant DNA technology. |
| EcoRV | Escherichia coli RV | GATATC | · Generates DNA fragments for mapping and analyzing restriction sites on DNA molecules. |
| NotI | Nocardia otitidis | GCGGCCGC | · Produces DNA fragments with defined ends thus facilities in precise mapping of DNA sequences. |
| SacI | Streptomyces achromogenes | GAGCTC | · Used to insert DNA fragments into expression vectors for studying gene function. |
| KpnI | Klebsiella pneumoniae | GGTACC | · It produces cohesive ends for ligation with compatible DNA fragments or vectors. |
| PstI | Providencia stuartii | CTGCAG | · It is often used in the digestion of DNA and for generating sticky ends. |
| SalI | Streptomyces albus G | GTCGAC | · This enzyme cuts DNA at specific sites, facilitating gene cloning and recombinant DNA construction. |