Past Papers Cell Biology

By: Prof. Fazal Rehman Shamil

Guess Paper 1: Cell Biology Fall – 2020 Past Papers

University Name – Confidential

Time Allowed: 3 hours

Total Marks:    70, Passing Marks (35)

Q.1 Mark the following statements either as True or False. (14 Marks)
a. Staining technique is a technique of using dyes like eosin, saffranine, Haematoxylin, fast green, methyleneblue to color the parts of cells. (True / False)
b. The protoplasm has a semi-fluid matrix called cytoplasm and a large membrane-bound structure called Nucleus. (True / False)
c. Prokaryotes have nucleus and other membrane-bound organelles. (True / False)
d. Prokaryotic cells always have cytoskeleton. (True / False)
e. Constituting 70–80 percent by weight of most cells, water is the most abundant molecule in biological systems. (True / False)
f. Many biomolecules (e.g., sugars) readily dissolve in water; these water-liking molecules are described as hydrophilic. (True / False)
g. Endoplasmic reticulum is a network of membrane-bound tubular structures in the cytoplasm. (True / False)
h. Endoplasmic reticulum is a storehouse of lipids and carbohydrates. (True / False)
i. Golgi complex has a group of curved, flattened plate like compartments called cisternae. (True / False)
j. Chromosomes are the structures that contain the genetic material. (True / False)
k. Cyclin-dependent kinases (Cdks) are inactive unless bound to cyclins. (True / False)
l. In anaphase, the paired chromosomes separate at the kinetochores and move to opposite sides of the cell. (True / False)
m. Signal transduction is defined as the response of a cell to the application of an external stimulus. (True / False)
n. The aim of cryopreservation is to reduce the risk of microbial contamination. (True / False)

Q.2 Compare prokaryotes and eukaryotes. (14 Marks)

Q.3 Write a detailed note on the cell membrane. (14 Marks)

Q.4 Describe mitochondria in detail. (14 Marks)

Q.5 Describe how cells are reproduced. (14 Marks)

Q.6 Describe apoptosis in detail. (14 Marks)

Q.7 Write chemical composition and molecular structure of eukaryotic chromosomes. (14 Marks)

Q.8 Write a short note on any two of the following: (14 Marks)
a) Cryopreservation of cell lines
b) Safety aspects of cell culture
c) Advantages of cell culture

Guess Paper 2: Cell Biology Spring – 2020 Past Papers

University Name – Confidential

Time Allowed: 3 hours

Total Marks:    70, Passing Marks (35)

Q.1 Mark the following statements either as True or False. (14 Marks)
a. Prokaryotic cells are smaller than eukaryotic cells. (True / False)
b. Prokaryote kingdom includes animals, plants, fungi and protoctista. (True / False)
c. The invention of electron microscope and staining techniques helped scientists to study the detailed structure of cell. (True / False)
d. A typical cell has an outer non living layer called cell wall. (True / False)
e. Centrioles are absent in plant cells. (True / False)
f. Endoplasmic reticulum helps in intracellular transportation. (True / False)
g. Golgi bodies produce Lysosomes. (True / False)
h. The bacterial chromosome is found in a region called the nucleoid. (True / False)
i. Chromosomes in eukaryotes are located in the cytoplasm. (True / False)
j. In the cell cycle, cells duplicate their contents and divide. (True / False)
k. Checkpoints in the cell cycle cannot ensure the cell cycle proceeds without errors. (True / False)
l. Spindle fibers line the chromosomes along the middle of the cell nucleus. This line is referred to as the metaphase plate. (True / False)
m. In biology, signal transduction refers to any process by which a cell converts one kind of signal or stimulus into another. (True / False)
n. Cryopreservation reduces the risk of microbial contamination. (True / False)

Q.2 Write a detailed note on prokaryotic cells. (14 Marks)

Q.3 Describe the lysosome in detail. (14 Marks)

Q.4 Write a detailed note on cell culture. (14 Marks)

Q.5 Describe meiosis in detail. (14 Marks)

Q.6 Describe signal transduction in detail. (14 Marks)

Q.7 Describe the cell cycle in detail. (14 Marks)

Q.8 Write a short note on any two of the following: (14 Marks)
a) Nucleosome
b) Heterochromatin
c) Euchromatin

Guess Paper 3: Cell Biology Spring – 2019 Past Papers

University Name – Confidential

Time Allowed: 3 hours

Total Marks:    70, Passing Marks (35)

Q.1 Mark the following statements either as True or False. (14 Marks)
i. In semi-conservative replication only half of the parental molecule is conserved during replication.T/ F
ii. Enzymes such as DNA polymerase and RNA polymerase are involved in the synthesis of nucleic acids by addition of bases to a growing nucleotide chain. (True / False)
iii. The DNA of eukaryotes is a long linear molecule with several replication units. (True / False)
iv. The synthesis of RNA uses the four ribonucleoside 5′-triphosphates (ATP, GTP, CTP, ATP) as precursors. (True / False)
v. The DNA sequence to which RNA polymerase binds to initiate transcription of a gene is called the promoter. (True / False)
vi. The single-stranded molecules of tRNA can fold on themselves forming hairpins and many of the bases become linked by H-bonds. (True / False)
vii. The genetic code for amino acids consists of triplets of bases. (True / False)
viii. Mutations that result from the influence of extraneous factors are considered to be induced mutations. (True / False)
ix. A change of multiple base pairs to disrupt open reading frame in a DNA molecule is known as a point mutation or base substitution. (True / False)
x. Nucleotide excision repair (NER) repairs “bulky” lesions in DNA that alter or distort the regular DNA double helix. (True / False)
xi. Fluorescent dyes are multicyclic molecules that absorb and emit fluorescent light at specific wavelengths. (True / False)
xii. Maxam-Gilbert sequencing is performed by chain breakage at specific nucleotides. (True / False)
xiii. A restriction enzyme recognizes and binds to DNA at a specific nucleotide sequence called a restriction site. (True / False)
xiv. Vectors are DNA molecules that accept DNA fragments and replicate inserted DNA fragments when vectors are placed into host cells. (True / False)

Q.2 Describe the molecular mechanism of replication. (14 Marks)

Q.3 Describe translation in detail. (14 Marks)

Q.4 Describe the regulation of gene expression in eukaryotes. (14 Marks)

Q.5 Describe mutations in detail. (14 Marks)

Q.6 Describe gene sequencing in detail. (14 Marks)

Q.7 Describe DNA damage and repair in detail. (14 Marks)

Q.8 Write a short note on any two of the following: (14 Marks)
a) Human Genome Project b) Stem cell research c) Bacterial plasmids.

Guess Paper 4: Cell Biology Fall – 2019 Past Papers

University Name – Confidential

Time Allowed: 3 hours

Total Marks:    70, Passing Marks (35)

Q.1 Mark the following statements either as True or False. (14 Marks)
a. E. coli replication begins from a fixed origin termed as oriC. (True / False)
b. DNA polymerase catalyzes chain growth in the 5′ → 3′ direction. (True / False)
c. Both replication and transcription use DNA as the template. (True / False)
d. DNA regions that can be transcribed into RNA are called structural genes. (True / False)
e. Shine-Dalgarno Sequence is involved in eukaryotic initiation of translation. (True / False)
f. Regulator genes encode products that regulate the expression of other genes. (True / False)
g. One or many promoters may occur for any given gene. (True / False)
h. Mutations are changes in DNA that do not affect genetic information. (True / False)
i. Mutations that result in complete loss of function are known as gain-of-function mutations. (True / False)
j. Most recognition sequences of restriction enzymes exhibit a form of symmetry described as a palindrome. (True / False)
k. Cosmids are hybrids of phages and plasmids. (True / False)
l. Fluorescent dyes are multicyclic molecules that absorb and emit fluorescent light at specific wavelengths. (True / False)
m. Stem cells do not have the ability to self-renew. (True / False)
n. A vector contains several restriction sites that allow the insertion of the DNA fragments to be cloned. (True / False)

Q.2 Describe the molecular mechanism of replication in eukaryotes. (14 Marks)

Q.3 Describe transcription in prokaryotes. (14 Marks)

Q.4 Write a detailed note on Lactose (lac) Operon in E. coli. (14 Marks)

Q.5 Describe types of mutations in detail. (14 Marks)

Q.6 Write a detailed note on vectors. (14 Marks)

Q.7 Write a detailed note on Chain Termination (Sanger) Sequencing. (14 Marks)

Q.8 Write a short note on any two of the following: (14 Marks)
a) Ethical, legal, and social issues of the human genome project
b) Embryonic stem cells
c) Photo repair

Prof. Fazal Rehman Shamil