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Biological Techniques Past Papers


Subject: Biological Techniques

Time Allowed: 15 Min

Total Marks: 10

NOTE: ATTEMPT THIS PAPER ON THIS QUESTION SHEET ONLY. Division of marks is given in front of each question. This Paper will be collected back after expiry of time limit mentioned above.


Part-I Encircle the correct option, each question carries equal marks. (10)

Part-I Encircle the correct option, each question carries equal marks. (10)
1. Gram staining is the example
a. Simple staining
b. Differential staining
c. Non differential staining
d. Capsular staining
2. The average size of RBC is
a. 68 µm
b. 1-l2 µm
c. 50 µm
d. 100 µm
3. Find the mode (34, 33, 34, 34, 35, 36, 33, 34, 31).
a. 33
b. 34
c. 35
d. 31
4. __________ inipurities can be separated by filtration.
a. Insoluble
b. Soluble and insoluble
c. Soluble
d. All type of impurities
5. The percentage of agarose for making of gel is chosen, based on which property of DNA
a. Shape
b. Charge
c. Size
d. All of these
6. ____________ is the running buffer for agarose gel.
a. Tris-acetate-EDTA
c. Ethidium bromide
d. All of these
7. .RNA is ________ stable than DNA
a. Less
b. More
c. Not known
d. Both a and b
8. Isopycnic is a type of technique called as
a. Distiflation
b. Ultracentrifugation
c. Filtration
d. None
9. The range of visible spectrum wavelength is
a. less than 400nm-700nm
b. more than 400nm-700nm
c. more than 1000nm
d. 400nm-700nm
10. ____________ is used as mounting material for slides in histology.
a. Formalin
b. Ethy! alcohol
c. Albumin
d. Ether
11. How thick are paraffin embedded sections that has been cut ?
a. 5-8uM
b. 580nM
c. 3-4mm
d. 3-4cm
12. What is the temperature of the paraffin the tissue is placed in ?
a. 76 c
b. 35 ¢
c. 56 c
d. 70 ¢
13. SI unit of area is
a. square meters
c. square yards
b. square inches
d. square feet
14. The thickness of a micron, is
a. 10-6 m
b. 10-3 m
c. 10-12 m
d. 10-9 m
15. The site in the cel! at which cellular respiration occurs is the ____________.
a. Endoplasmic reticulum
b. Golgi complex
c. Ribosome
d. Mitochondria
16. In gel electrophoresis, how do we make the DNA migrate through the gel?
a. We place a negative electrode away from the wells
b. Gravity
c. Large fragments drift to the end of the gel
d. We place a positive electrode away from the wells
17. What do we use to cut the DNA before gel electrophoresis?
a. Lipase
b. Restriction enzymes
c. Helicase
d. DNA polymerase
18. Which is NOT a reason for using gel electrophoresis?
a. Organizing DNA by the shape of the backbone
b. Organizing DNA fragments from largest to smallest
c. Comparing two sets of DNA
d. Organizing DNA in an order that we can see
19. fluorescent dye such Ethidium is used for visualizing DNA. How does ethidium bind to DNA?
a. Stacked between histone molecules
b. Binds to the nucleotide base
c. Intercalated between the stacked bases
d. Binds to the phosphodiester backbone
20. Which of the following components on a light microscope should be focused first?
a. The two eye pieces
b. Objective lenses
c. Condenser
d. Revolving nose piece


Subject: Biological Techniques

Time Allowed: 2 Hour 45 Min

Total Marks: 50



Part-II Give short answers, each question carries equal marks. (20)

Q#1: Calculate the magnification of a microscope (ocular lens=20x, objective lens=40x).

Q#2: Which embedding material is most commonly used in histological techniques?

Q#3: Differentiate between mobile and stationary phases in chromatography.

Q#4: Enlist some important active techniques for invertebrate sampling.

Q#5: What is colorimetry? How it is useful in laboratories.

Q#6: Does density gradient centrifugation play any role in cell fractionation?

Q#7: Distinguish between the visible light spectrum and the ultraviolet spectrum.

Q#8: Define the least count-off screw gauge.

Q#9: Why is staining used for tissue study?

Q#10: Write the working principle of thin-layer chromatography.


Part-III Give detailed answers, each question carries equal marks. (30)

Q#1: What are the purpose and procedure of haematoxylin and eosin staining?

Q#2: Write a comprehensive note on agarose gel electrophoresis.

Q#3: How to write a research paper.

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